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Monoclonal antibody 2C5 specifically targets neutrophil extracellular traps

Authors :
Livia P. Mendes
Kobra Rostamizadeh
Kandace Gollomp
Jacob W. Myerson
Oscar A. Marcos-Contreras
Marco Zamora
Ed Luther
Jacob S. Brenner
Nina Filipczak
Xiang Li
Vladimir P. Torchilin
Source :
mAbs, Vol 12, Iss 1 (2020)
Publication Year :
2020
Publisher :
Taylor & Francis Group, 2020.

Abstract

Neutrophils can release DNA and granular cytoplasmic proteins that form smooth filaments of stacked nucleosomes (NS). These structures, called neutrophil extracellular traps (NETs), are involved in multiple pathological processes, and NET formation and removal are clinically significant. The monoclonal antibody 2C5 has strong specificity toward intact NS but not to individual NS components, indicating that 2C5 could potentially target NS in NETs. In this study, NETs were generated in vitro using neutrophils and HL-60 cells differentiated into granulocyte-like cells. The specificity of 2C5 toward NETs was evaluated by ELISA, which showed that it binds to NETs with the specificity similar to that for purified nucleohistone substrate. Immunofluorescence showed that 2C5 stains NETs in both static and perfused microfluidic cell cultures, even after NET compaction. Modification of liposomes with 2C5 dramatically enhanced liposome association with NETs. Our results suggest that 2C5 could be used to identify and visualize NETs and serve as a ligand for NET-targeted diagnostics and therapies.

Details

Language :
English
ISSN :
19420862 and 19420870
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
mAbs
Publication Type :
Academic Journal
Accession number :
edsdoj.8d2f118ff53140ff9423e891e3e3516f
Document Type :
article
Full Text :
https://doi.org/10.1080/19420862.2020.1850394