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On the fluorescence enhancement of arch neuronal optogenetic reporters

Authors :
Leonardo Barneschi
Emanuele Marsili
Laura Pedraza-González
Daniele Padula
Luca De Vico
Danil Kaliakin
Alejandro Blanco-González
Nicolas Ferré
Miquel Huix-Rotllant
Michael Filatov
Massimo Olivucci
Source :
Nature Communications, Vol 13, Iss 1, Pp 1-9 (2022)
Publication Year :
2022
Publisher :
Nature Portfolio, 2022.

Abstract

Arch-3 rhodopsin variants are common fluorescent reporters of neuronal activity. Here, the authors show with quantum chemical modelling that a set of these proteins reveals a direct proportionality between their observed fluorescence intensity and the stability of an exotic excited-state diradical intermediate.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
13
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.8db7a882c81c4841b40e3170f489ada1
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-022-33993-4