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CRISPR-Cas-Based Engineering of Probiotics
- Source :
- BioDesign Research, Vol 5 (2023)
- Publication Year :
- 2023
- Publisher :
- American Association for the Advancement of Science (AAAS), 2023.
-
Abstract
- Probiotics are the treasure of the microbiology fields. They have been widely used in the food industry, clinical treatment, and other fields. The equivocal health-promoting effects and the unknown action mechanism were the largest obstacles for further probiotic’s developed applications. In recent years, various genome editing techniques have been developed and applied to explore the mechanisms and functional modifications of probiotics. As important genome editing tools, CRISPR-Cas systems that have opened new improvements in genome editing dedicated to probiotics. The high efficiency, flexibility, and specificity are the advantages of using CRISPR-Cas systems. Here, we summarize the classification and distribution of CRISPR-Cas systems in probiotics, as well as the editing tools developed on the basis of them. Then, we discuss the genome editing of probiotics based on CRISPR-Cas systems and the applications of the engineered probiotics through CRISPR-Cas systems. Finally, we proposed a design route for CRISPR systems that related to the genetically engineered probiotics.
- Subjects :
- Biotechnology
TP248.13-248.65
Genetics
QH426-470
Subjects
Details
- Language :
- English
- ISSN :
- 26931257
- Volume :
- 5
- Database :
- Directory of Open Access Journals
- Journal :
- BioDesign Research
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.8dda960c75a4601bd2aaaa6227a9109
- Document Type :
- article
- Full Text :
- https://doi.org/10.34133/bdr.0017