Effects of insulin on proliferation and secretion of transforming growth factor-β2 of human retinal pigment epithelial cells

AIM: To investigate if insulin can promote retinal pigment epithelial(RPE)cell proliferation and the secretion of transforming growth factor β2(TGF-β2). METHODS: Human RPE cells were exposed to different concentrations insulin at different times(0 to 48 hours). We used the MTS method to test the proliferation of RPE cells. And we used the ELISA method to text the secretion of TGF-β2 of RPE cells in various cases, and the Real-time PCR method to test the expressions of TGF-β2 mRNA. RESULTS: The MTS results showed that after 12 hours exposure of insulin RPE cells proliferated significantly(P