Back to Search Start Over

Heterogeneous IL-9 Production by Circulating Skin-Tropic and Extracutaneous Memory T Cells in Atopic Dermatitis Patients

Authors :
Irene García-Jiménez
Lídia Sans-de San Nicolás
Laia Curto-Barredo
Marta Bertolín-Colilla
Eloi Sensada-López
Ignasi Figueras-Nart
Montserrat Bonfill-Ortí
Antonio Guilabert-Vidal
Anna Ryzhkova
Marta Ferran
Giovanni Damiani
Tali Czarnowicki
Ramon M. Pujol
Luis F. Santamaria-Babí
Source :
International Journal of Molecular Sciences, Vol 25, Iss 16, p 8569 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Interleukin (IL)-9 is present in atopic dermatitis (AD) lesions and is considered to be mainly produced by skin-homing T cells expressing the cutaneous lymphocyte-associated antigen (CLA). However, its induction by AD-associated triggers remains unexplored. Circulating skin-tropic CLA+ and extracutaneous/systemic CLA− memory T cells cocultured with autologous lesional epidermal cells from AD patients were activated with house dust mite (HDM) and staphylococcal enterotoxin B (SEB). Levels of AD-related mediators in response to both stimuli were measured in supernatants, and the cytokine response was associated with different clinical characteristics. Both HDM and SEB triggered heterogeneous IL-9 production by CLA+ and CLA− T cells in a clinically homogenous group of AD patients, which enabled patient stratification into IL-9 producers and non-producers, with the former group exhibiting heightened HDM-specific and total IgE levels. Upon allergen exposure, IL-9 production depended on the contribution of epidermal cells and class II-mediated presentation; it was the greatest cytokine produced and correlated with HDM-specific IgE levels, whereas SEB mildly induced its release. This study demonstrates that both skin-tropic and extracutaneous memory T cells produce IL-9 and suggests that the degree of allergen sensitization reflects the varied IL-9 responses in vitro, which may allow for patient stratification in a clinically homogenous population.

Details

Language :
English
ISSN :
14220067 and 16616596
Volume :
25
Issue :
16
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.8e9681619474f5ca81510dad6234796
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms25168569