Back to Search Start Over

Molecular cytogenetic characterization and fusarium head blight resistance of five wheat-Thinopyrum intermedium partial amphiploids

Authors :
Hui Wang
Shuwei Cheng
Yue Shi
Shuxin Zhang
Wei Yan
Weifu Song
Xuefeng Yang
Qingjie Song
Bo Jang
Xiaoyue Qi
Xinling Li
Bernd Friebe
Yanming Zhang
Source :
Molecular Cytogenetics, Vol 14, Iss 1, Pp 1-11 (2021)
Publication Year :
2021
Publisher :
BMC, 2021.

Abstract

Abstract Background Partial amphiploids created by crossing octoploid tritelytrigia(2n = 8× = 56, AABBDDEE) and Thinopyrum intermedium (2n = 6× = 42, StStJJJSJS) are important intermediates in wheat breeding because of their resistance to major wheat diseases. We examined the chromosome compositions of five wheat-Th. intermedium partial amphiploids using GISH and multicolor-FISH. Results The result revealed that five lines had 10-14 J-genome chromosomes from Th. intermedium and 42 common wheat chromosomes, using the J-genomic DNA from Th. bessarabicum as GISH probe and the oligo probes pAs1-1, pAs1-3, AFA-4, (GAA) 10, and pSc119.2-1 as FISH probe. Five lines resembled their parent octoploid tritelytrigia (2n = 8× = 56, AABBDDEE) but had higher protein contents. Protein contents of two lines HS2-2 and HS2-5 were up to more than 20%. Evaluation of Fusarium head blight (FHB) resistance revealed that the percent of symptomatic spikelets (PSS) of these lines were below 30%. Lines HS2-2, HS2-4, HS2-5, and HS2-16 were less than 20% of PPS. Line HS2-5 with 14 J-genome chromosomes from Th. intermedium showed the best disease resistance, with PSS values of 10.8% and 16.6% in 2016 and 2017, respectively. Conclusions New wheat-Th. intermedium amphiploids with the J-genome chromosomes were identified and can be considered as a valuable source of FHB resistance in wheat breeding.

Details

Language :
English
ISSN :
17558166
Volume :
14
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Molecular Cytogenetics
Publication Type :
Academic Journal
Accession number :
edsdoj.8e9b32a31b624ae28a22dfb4ce51bb1e
Document Type :
article
Full Text :
https://doi.org/10.1186/s13039-021-00536-3