Back to Search Start Over

RNA polymerase II transcription initiation in holo-TFIID-depleted mouse embryonic stem cells

Authors :
Vincent Hisler
Paul Bardot
Dylane Detilleux
Andrea Bernardini
Matthieu Stierle
Emmanuel Garcia Sanchez
Claire Richard
Lynda Hadj Arab
Cynthia Ehrhard
Bastien Morlet
Yavor Hadzhiev
Matthieu Jung
Stéphanie Le Gras
Luc Négroni
Ferenc Müller
László Tora
Stéphane D. Vincent
Source :
Cell Reports, Vol 43, Iss 10, Pp 114791- (2024)
Publication Year :
2024
Publisher :
Elsevier, 2024.

Abstract

Summary: The recognition of core promoter sequences by TFIID is the first step in RNA polymerase II (Pol II) transcription initiation. Metazoan holo-TFIID is a trilobular complex, composed of the TATA binding protein (TBP) and 13 TBP-associated factors (TAFs). Why and how TAFs are necessary for the formation of TFIID domains and how they contribute to transcription initiation remain unclear. Inducible TAF7 or TAF10 depletion, followed by comprehensive analysis of TFIID subcomplex formation, chromatin binding, and nascent transcription in mouse embryonic stem cells, result in the formation of a TAF7-lacking TFIID or a minimal core-TFIID complex, respectively. These partial complexes support TBP recruitment at promoters and nascent Pol II transcription at most genes early after depletion, but importantly, TAF10 is necessary for efficient Pol II pausing. We show that partially assembled TFIID complexes can sustain Pol II transcription initiation but cannot replace holo-TFIID over several cell divisions and/or development.

Details

Language :
English
ISSN :
22111247
Volume :
43
Issue :
10
Database :
Directory of Open Access Journals
Journal :
Cell Reports
Publication Type :
Academic Journal
Accession number :
edsdoj.8f578fb5cc1f402db296167faf6950f2
Document Type :
article
Full Text :
https://doi.org/10.1016/j.celrep.2024.114791