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Highly Sensitive Love Mode Acoustic Wave Platform with SiO2 Wave-Guiding Layer and Gold Nanoparticles for Detection of Carcinoembryonic Antigens
- Source :
- Biosensors, Vol 12, Iss 7, p 536 (2022)
- Publication Year :
- 2022
- Publisher :
- MDPI AG, 2022.
-
Abstract
- A highly sensitive and precise Love wave mode surface acoustic wave (SAW) immunosensor based on an ST-cut 90°X quartz substrate and an SiO2 wave-guiding layer was developed to detect cancer-related biomarkers of carcinoembryonic antigens (CEAs). A delay line structure of the SAW device with a resonant frequency of 196 MHz was designed/fabricated, and its surface was functionalized through CEA antibody immobilization. The CEA antibodies were bound with gold nanoparticles and CEA antibodies to form a sandwich structure, which significantly amplified the mass loading effect and enhanced the maximum responses by 30 times. The center frequency of the Love wave immunosensor showed a linear response as a function of the CEA concentration in the range of 0.2–5 ng/mL. It showed a limit of detection of 0.2 ng/mL, and its coefficient of determination was 0.983. The sensor also showed minimal interference from nonspecific adsorptions, thus demonstrating its promise for point-of-care applications for cancer biomarkers.
- Subjects :
- love wave
biosensors
CEA
AuNPs
Biotechnology
TP248.13-248.65
Subjects
Details
- Language :
- English
- ISSN :
- 20796374
- Volume :
- 12
- Issue :
- 7
- Database :
- Directory of Open Access Journals
- Journal :
- Biosensors
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.8ff167d17a764004a15dbe6829abf9f6
- Document Type :
- article
- Full Text :
- https://doi.org/10.3390/bios12070536