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Highly Sensitive Love Mode Acoustic Wave Platform with SiO2 Wave-Guiding Layer and Gold Nanoparticles for Detection of Carcinoembryonic Antigens

Authors :
Chong Li
Jikai Zhang
Haiyu Xie
Jingting Luo
Chen Fu
Ran Tao
Honglang Li
Yongqing Fu
Source :
Biosensors, Vol 12, Iss 7, p 536 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

A highly sensitive and precise Love wave mode surface acoustic wave (SAW) immunosensor based on an ST-cut 90°X quartz substrate and an SiO2 wave-guiding layer was developed to detect cancer-related biomarkers of carcinoembryonic antigens (CEAs). A delay line structure of the SAW device with a resonant frequency of 196 MHz was designed/fabricated, and its surface was functionalized through CEA antibody immobilization. The CEA antibodies were bound with gold nanoparticles and CEA antibodies to form a sandwich structure, which significantly amplified the mass loading effect and enhanced the maximum responses by 30 times. The center frequency of the Love wave immunosensor showed a linear response as a function of the CEA concentration in the range of 0.2–5 ng/mL. It showed a limit of detection of 0.2 ng/mL, and its coefficient of determination was 0.983. The sensor also showed minimal interference from nonspecific adsorptions, thus demonstrating its promise for point-of-care applications for cancer biomarkers.

Details

Language :
English
ISSN :
20796374
Volume :
12
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Biosensors
Publication Type :
Academic Journal
Accession number :
edsdoj.8ff167d17a764004a15dbe6829abf9f6
Document Type :
article
Full Text :
https://doi.org/10.3390/bios12070536