High-Throughput Array Production Using Precision Glass Syringes

Authors :: J. Shieh
C. To
J. Carramao
N. Nishimura
Y. Maruta
Y. Hashimoto
D. Wright
H.-c. Wu
A. Azarani
Source :: BioTechniques, Vol 32, Iss 6, Pp 1360-1365 (2002)
Publication Year :: 2002
Publisher :: Taylor & Francis Group, 2002.
Abstract: The advantages of using 1, 96, or 384 precision glass syringes in automated high-throughput microdispensers in creating highly uniform and reproducible DNA, protein, and organic compound array filters and slides are described. Using the Hydra® Microdispenser and TangoTM Liquid Handling system, 0.1–5 ng (in 50–300 nL) PCR-amplified, human cancer-related genes and housekeeping genes were spotted onto nylon membranes and coated slides. Protein solutions of 50μg/mL to 1 mg/mL were spotted onto coated slides or onto MaxiSorpTM 96-well plates. Up to 6144 spots/membrane and up to 1000 spots/slide were printed. The size of the spots created by glass syringes was uniform and reproducible (precision variation of less than 5%) from spot to spot and membrane to membrane. Using a Tango 384 system, a total of ten 6144-spot filters can be produced in approximately 25 min, translating into a spotting speed of 2.5 min/membrane.