Tubeimoside-1 affects the phenotype of skin squamous cell carcinoma SCL-1 cells by regulating the circ_0000376/miR-203 axis

Objective: To investigate the effect of tubeimoside-1 on the phenotype of skin squamous cell carcinoma SCL-1 cells and possible mechanism of action. Methods: SCL-1 cells were divided into the control group and the treatment groups, including different doses (5, 10, 20 μg/mL) of tubeimoside-1 groups, si-NC group, si-circ_0000376 group, tubeimoside-1+ pcDNA group, and tubeimoside-1+pcDNA-circ_0000376 group. CCK-8 method was used to detect cell proliferation inhibition rate.Transwell was used to detect cell migration and invasion. Flow cytometry was used to detect cell apoptosis. Western blot was used to detect the protein expression of Ki-67, MMP-2, MMP-9, Bcl-2 and Bax. RT-qPCR method was used to detect the expression of circ_0000376 and miR-203. The dual luciferase reporter gene experiment was used to verify the regulatory relationship between circ_0000376 and miR-203. Results: Compared with the control group, the proliferation inhibition rate, apoptosis rate and the Bax protein expression of cells in the tubeimoside-1 group were significantly increased (F=772.61, 352.20, 277.56, all P