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Methods for shipping live primary cortical and hippocampal neuron cultures from postnatal mice

Authors :
Ferass M. Sammoura
Dina Popova
Ayeshia Morris
Ronald P. Hart
Jason R. Richardson
Source :
Current Research in Neurobiology, Vol 4, Iss , Pp 100069- (2023)
Publication Year :
2023
Publisher :
Elsevier, 2023.

Abstract

Primary neuronal cultures have proven to be a powerful tool for studying mechanisms in neuroscience. It is technically challenging and expensive to reproduce high quality viable neuronal cultures. Laboratories that are not experienced or equipped to prepare primary neuron cultures may have difficulty producing consistent cultures for experiments. It has previously been shown that live rat embryonic hippocampal cultures can be shipped from laboratories that produce them. Here, we show that variations to this procedure allow for shipping postnatal mouse cultures of hippocampal and cortical primary neurons using standard commercial couriers. We also show that after shipping, primary neurons are viable, express synaptic markers, and demonstrate physiological activity, making them relevant models over immortalized cell lines. Among the many applications of this technique would be the preparation of cultured neurons from transgenic mouse lines in one laboratory and sharing them with distant collaborators, reducing variability.

Details

Language :
English
ISSN :
2665945X
Volume :
4
Issue :
100069-
Database :
Directory of Open Access Journals
Journal :
Current Research in Neurobiology
Publication Type :
Academic Journal
Accession number :
edsdoj.925d087ab8746e8a3786a1a3d8bc932
Document Type :
article
Full Text :
https://doi.org/10.1016/j.crneur.2022.100069