Back to Search Start Over

Complete Genome Sequence of Herpes Simplex Virus 2 Strain G

Authors :
Weizhong Chang
Xiaoli Jiao
Hongyan Sui
Suranjana Goswami
Brad T. Sherman
Caroline Fromont
Juan Manuel Caravaca
Bao Tran
Tomozumi Imamichi
Source :
Viruses, Vol 14, Iss 3, p 536 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Herpes simplex virus type 2 (HSV-2) is a common causative agent of genital tract infections. Moreover, HSV-2 and HIV infection can mutually increase the risk of acquiring another virus infection. Due to the high GC content and highly repetitive regions in HSV-2 genomes, only the genomes of four strains have been completely sequenced (HG52, 333, SD90e, and MS). Strain G is commonly used for HSV-2 research, but only a partial genome sequence has been assembled with Illumina sequencing reads. In the current study, we de novo assembled and annotated the complete genome of strain G using PacBio long sequencing reads, which can span the repetitive regions, analyzed the ‘α’ sequence, which plays key roles in HSV-2 genome circulation, replication, cleavage, and packaging of progeny viral DNA, identified the packaging signals homologous to HSV-1 within the ‘α’ sequence, and determined both termini of the linear genome and cleavage site for the process of concatemeric HSV-2 DNA produced via rolling-circle replication. In addition, using Oxford Nanopore Technology sequencing reads, we visualized four HSV-2 genome isomers at the nucleotide level for the first time. Furthermore, the coding sequences of HSV-2 strain G have been compared with those of HG52, 333, and MS. Moreover, phylogenetic analysis of strain G and other diverse HSV-2 strains has been conducted to determine their evolutionary relationship. The results will aid clinical research and treatment development of HSV-2.

Details

Language :
English
ISSN :
19994915
Volume :
14
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Viruses
Publication Type :
Academic Journal
Accession number :
edsdoj.9323458826054e8b8e135be82855170f
Document Type :
article
Full Text :
https://doi.org/10.3390/v14030536