Antimicrobial Effect of Lactobacillus kunkeei Against Pathogenic Bacteria Isolated from Bees’ Gut

The aim of this study was to evaluate antimicrobial activity of Lactobacillus kunkeei of bee’s gastrointestinal tract (GUT) against pathogenic bacteria isolated from bees’ gut. Content of the intestinal tract microbiota was cultured for isolation of different group of bacteria. Gut homogenates were plated on de Man, Rogosa and Sharpe agar plates and incubated for 48-72h at 30 °C anaerobically; on McConkey agar and incubated for 24-48h at 37°C aerobically. Then, the identification of isolates with MALDI-TOF MS Biotyper was done. The bacterial strains of Lactobacillus kunkeei, Bacillus cereus, Bacillus megatherium, Bacillus simplex, Bacillus thuringiensis, Delfia acidovorans, Escherichia coli, Enterococcus faecalis, Klebsiella aerogenes, Morganella morganii, Pseudomonas aeruginosa, Serratia marcescens and Sphingomonas parapaucimobilis were isolated from gut content of bees. The disc diffusion method was used for the determination of antimicrobial activities of the Lactobacillus kunkeei supernatant against other isolated bacterial strains of bees’ origin. The best antimicrobial activity of Lactobacillus kunkeei supernatant was found against Klebsiella aerogenes, Morganella morganii, Pseudomonas aeruginosa, Serratia marcescens. Less effective the L. kunkeei supernatant was against Bacillus cereus, Bacillus megatherium, Bacillus simplex, Bacillus thuringiensis. The moderate antibacterial activity was identified against Delfia acidovorans, Escherichia coli, Enterococcus faecalis, Sphingomonas parapaucimobilis.