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Qing Hua Chang Yin ameliorates chronic colitis in mice by inhibiting PERK–ATF4–CHOP pathway of ER stress and the NF-κB signalling pathway

Authors :
Liya Liu
Youqin Chen
Yuying Han
Xinran Zhang
Yulun Wu
Jing Lin
Liujing Cao
Meizhu Wu
Huifang Zheng
Yi Fang
Lihui Wei
Thomas J. Sferra
Anjum Jafri
Xiao Ke
Jun Peng
Aling Shen
Source :
Pharmaceutical Biology, Vol 62, Iss 1, Pp 607-620 (2024)
Publication Year :
2024
Publisher :
Taylor & Francis Group, 2024.

Abstract

Context Ulcerative colitis has been clinically treated with Qing Hua Chang Yin (QHCY), a traditional Chinese medicine formula. However, its precise mechanisms in mitigating chronic colitis are largely uncharted.Objective To elucidate the therapeutic efficiency of QHCY on chronic colitis and explore its underlying molecular mechanisms.Materials and methods A total ion chromatogram fingerprint of QHCY was analysed. Chronic colitis was induced in male C57BL/6 mice using 2% dextran sodium sulphate (DSS) over 49 days. Mice were divided into control, DSS, DSS + QHCY (0.8, 1.6 and 3.2 g/kg/d dose, respectively) and DSS + mesalazine (0.2 g/kg/d) groups (n = 6). Mice were intragastrically administered QHCY or mesalazine for 49 days. The changes of disease activity index (DAI), colon length, colon histomorphology and serum pro-inflammatory factors in mice were observed. RNA sequencing was utilized to identify the differentially expressed transcripts (DETs) in colonic tissues and the associated signalling pathways. The expression of endoplasmic reticulum (ER) stress-related protein and NF-κB signalling pathway-related proteins in colonic tissues was detected by immunohistochemistry staining.Results Forty-seven compounds were identified in QHCY. Compared with the DSS group, QHCY significantly improved symptoms of chronic colitis like DAI increase, weight loss, colon shortening and histological damage. It notably reduced serum levels of IL-6, IL-1β and TNF-α. QHCY suppressed the activation of PERK–ATF4–CHOP pathway of ER stress and NF-κB signalling pathways in colonic tissues.Discussion and conclusions The findings in this study provide novel insights into the potential of QHCY in treating chronic colitis patients.

Details

Language :
English
ISSN :
13880209 and 17445116
Volume :
62
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Pharmaceutical Biology
Publication Type :
Academic Journal
Accession number :
edsdoj.942f776fc7894a3895d95242b37c2669
Document Type :
article
Full Text :
https://doi.org/10.1080/13880209.2024.2378012