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Portable and Label-Free Quantitative Loop-Mediated Isothermal Amplification (LF-qLamp) for Reliable COVID-19 Diagnostics in Three Minutes of Reaction Time: Arduino-Based Detection System Assisted by a pH Microelectrode

Portable and Label-Free Quantitative Loop-Mediated Isothermal Amplification (LF-qLamp) for Reliable COVID-19 Diagnostics in Three Minutes of Reaction Time: Arduino-Based Detection System Assisted by a pH Microelectrode

Authors :
Mario Moisés Alvarez
Sergio Bravo-González
Everardo González-González
Grissel Trujillo-de Santiago
Source :
Biosensors, Vol 11, Iss 10, p 386 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

Loop-mediated isothermal amplification (LAMP) has been recently studied as an alternative method for cost-effective diagnostics in the context of the current COVID-19 pandemic. Recent reports document that LAMP-based diagnostic methods have a comparable sensitivity and specificity to that of RT-qPCR. We report the use of a portable Arduino-based LAMP-based amplification system assisted by pH microelectrodes for the accurate and reliable diagnosis of SARS-CoV-2 during the first 3 min of the amplification reaction. We show that this simple system enables a straightforward discrimination between samples containing or not containing artificial SARS-CoV-2 genetic material in the range of 10 to 10,000 copies per 50 µL of reaction mix. We also spiked saliva samples with SARS-CoV-2 synthetic material and corroborated that the LAMP reaction can be successfully monitored in real time using microelectrodes in saliva samples as well. These results may have profound implications for the design of real-time and portable quantitative systems for the reliable detection of viral pathogens including SARS-CoV-2.

Details

Language :
English
ISSN :
20796374
Volume :
11
Issue :
10
Database :
Directory of Open Access Journals
Journal :
Biosensors
Publication Type :
Academic Journal
Accession number :
edsdoj.94f6d952b472290e69b31779493f4
Document Type :
article
Full Text :
https://doi.org/10.3390/bios11100386