Back to Search Start Over

Optical detection of three modes of endocytosis at hippocampal synapses

Authors :
Natali L Chanaday
Ege T Kavalali
Source :
eLife, Vol 7 (2018)
Publication Year :
2018
Publisher :
eLife Sciences Publications Ltd, 2018.

Abstract

Coupling of synaptic vesicle fusion and retrieval constitutes a core mechanism ensuring maintenance of presynaptic function. Recent studies using fast-freeze electron microscopy and capacitance measurements reported an ultrafast mode of endocytosis operating at physiological temperatures. Here, using rat hippocampal neurons, we optically monitored single synaptic vesicle endocytosis with high time resolution using the vesicular glutamate transporter, synaptophysin and the V0a1 subunit of the vacuolar ATPase as probes. In this setting, we could distinguish three components of retrieval operating at ultrafast (~150–250 ms, ~20% of events), fast (~5–12 s, ~40% of events) and ultraslow speeds (>20 s, ~40% of events). While increasing Ca2+ slowed the fast events, increasing temperature accelerated their time course. In contrast, the kinetics of ultrafast events were only mildly affected by these manipulations. These results suggest that synaptic vesicle proteins can be retrieved with ultrafast kinetics, although a majority of evoked fusion events are coupled to slower retrieval mechanisms.

Details

Language :
English
ISSN :
2050084X
Volume :
7
Database :
Directory of Open Access Journals
Journal :
eLife
Publication Type :
Academic Journal
Accession number :
edsdoj.953a3e72915b4a76bd5e0ae6eb587536
Document Type :
article
Full Text :
https://doi.org/10.7554/eLife.36097