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A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices
A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices
- Source :
- STAR Protocols, Vol 2, Iss 2, Pp 100527- (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Summary: Small molecular probes designed for photopharmacology and opto-chemogenetics are rapidly gaining widespread recognition for investigations of transient receptor potential canonical (TRPC) channels. This protocol describes the use of three photoswitchable diacylglycerol analogs—PhoDAG-1, PhoDAG-3, and OptoDArG—for ultrarapid activation and deactivation of native TRPC2 channels in mouse vomeronasal sensory neurons and olfactory type B cells, as well as heterologously expressed human TRPC6 channels. Photoconversion can be achieved in mammalian tissue slices and enables all-optical stimulation and shutoff of TRPC channels.For complete details on the use and execution of this protocol, please refer to Leinders-Zufall et al. (2018).
Details
- Language :
- English
- ISSN :
- 26661667
- Volume :
- 2
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- STAR Protocols
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.971563504d542199dde374703f4cf03
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.xpro.2021.100527