Back to Search Start Over

A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices

A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices

Authors :
Trese Leinders-Zufall
Ursula Storch
Michael Mederos y Schnitzler
Navin K. Ojha
Kohei Koike
Thomas Gudermann
Frank Zufall
Source :
STAR Protocols, Vol 2, Iss 2, Pp 100527- (2021)
Publication Year :
2021
Publisher :
Elsevier, 2021.

Abstract

Summary: Small molecular probes designed for photopharmacology and opto-chemogenetics are rapidly gaining widespread recognition for investigations of transient receptor potential canonical (TRPC) channels. This protocol describes the use of three photoswitchable diacylglycerol analogs—PhoDAG-1, PhoDAG-3, and OptoDArG—for ultrarapid activation and deactivation of native TRPC2 channels in mouse vomeronasal sensory neurons and olfactory type B cells, as well as heterologously expressed human TRPC6 channels. Photoconversion can be achieved in mammalian tissue slices and enables all-optical stimulation and shutoff of TRPC channels.For complete details on the use and execution of this protocol, please refer to Leinders-Zufall et al. (2018).

Details

Language :
English
ISSN :
26661667
Volume :
2
Issue :
2
Database :
Directory of Open Access Journals
Journal :
STAR Protocols
Publication Type :
Academic Journal
Accession number :
edsdoj.971563504d542199dde374703f4cf03
Document Type :
article
Full Text :
https://doi.org/10.1016/j.xpro.2021.100527