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Two-colour live-cell nanoscale imaging of intracellular targets

Authors :
Francesca Bottanelli
Emil B. Kromann
Edward S. Allgeyer
Roman S. Erdmann
Stephanie Wood Baguley
George Sirinakis
Alanna Schepartz
David Baddeley
Derek K. Toomre
James E. Rothman
Joerg Bewersdorf
Source :
Nature Communications, Vol 7, Iss 1, Pp 1-5 (2016)
Publication Year :
2016
Publisher :
Nature Portfolio, 2016.

Abstract

The intracellular applications of STED microscopy are limited by the availability of dyes. Here the authors develop a two-colour labelling strategy based on SiR and ATTO590 dyes, and apply their strategy to image various subcellular membrane compartments.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
7
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.973638e80347418c454d3d0a47cc6b
Document Type :
article
Full Text :
https://doi.org/10.1038/ncomms10778