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Analysis of endophytic bacterial diversity in seeds of different genotypes of cotton and the suppression of Verticillium wilt pathogen infection by a synthetic microbial community

Authors :
Chong-Die Wu
Yong-Bin Fan
Xue Chen
Jiang-Wei Cao
Jing-Yi Ye
Meng-Lei Feng
Xing-Xing Liu
Wen-Jing Sun
Rui-Na Liu
Ai-Ying Wang
Source :
BMC Plant Biology, Vol 24, Iss 1, Pp 1-15 (2024)
Publication Year :
2024
Publisher :
BMC, 2024.

Abstract

Abstract Background In agricultural production, fungal diseases significantly impact the yield and quality of cotton (Gossypium spp.) with Verticillium wilt posing a particularly severe threat. Results This study is focused on investigating the effectiveness of endophytic microbial communities present in the seeds of disease-resistant cotton genotypes in the control of cotton Verticillium wilt. The technique of 16S ribosomal RNA (16S rRNA) amplicon sequencing identified a significant enrichment of the Bacillus genus in the resistant genotype Xinluzao 78, which differed from the endophytic bacterial community structure in the susceptible genotype Xinluzao 63. Specific enriched strains were isolated and screened from the seeds of Xinluzao 78 to further explore the biological functions of seed endophytes. A synthetic microbial community (SynCom) was constructed using the broken-rod model, and seeds of the susceptible genotype Xinluzao 63 in this community that had been soaked with the SynCom were found to significantly control the occurrence of Verticillium wilt and regulate the growth of cotton plants. Antibiotic screening techniques were used to preliminarily identify the colonization of strains in the community. These techniques revealed that the strains can colonize plant tissues and occupy ecological niches in cotton tissues through a priority effect, which prevents infection by pathogens. Conclusion This study highlights the key role of seed endophytes in driving plant disease defense and provides a theoretical basis for the future application of SynComs in agriculture.

Details

Language :
English
ISSN :
14712229
Volume :
24
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Plant Biology
Publication Type :
Academic Journal
Accession number :
edsdoj.99200ebacb704456a3792e1327b14410
Document Type :
article
Full Text :
https://doi.org/10.1186/s12870-024-04910-2