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Efficient secretory expression of phospholipase D for the high-yield production of phosphatidylserine and phospholipid derivates from soybean lecithin

Authors :
Peng Zhang
Jin-Song Gong
Zhi-Hao Xie
Chang Su
Xiao-Mei Zhang
Zhi-Ming Rao
Zheng-Hong Xu
Jin-Song Shi
Source :
Synthetic and Systems Biotechnology, Vol 8, Iss 2, Pp 273-280 (2023)
Publication Year :
2023
Publisher :
KeAi Communications Co., Ltd., 2023.

Abstract

Phospholipase D (PLD) is an essential biocatalyst for the biological production of phosphatidylserine and phospholipid modification. However, the efficient heterologous expression of PLD is limited by its cell toxicity. In this study, a PLD was secretory expressed efficiently in Bacillus subtilis with an activity around 100 U/mL. A secretory expression system containing the signal peptide SPEstA and the dual-promoter PHpaII-SrfA was established, and the extracellular PLD activity further reached 119.22 U/mL through scale-up fermentation, 191.30-fold higher than that of the control. Under optimum reaction conditions, a 61.61% conversion ratio and 21.07 g/L of phosphatidylserine production were achieved. Finally, the synthesis system of PL derivates was established, which could efficiently synthesis novel PL derivates. The results highlight that the secretory expression system constructed in this study provides a promising PLD producing strain in industrial application, and laid the foundation for the biosynthesis of phosphatidylserine and other PL derivates. As far as we know, this work reports the highest level of extracellular PLD expression to date and the enzymatic production of several PL derivates for the first time.

Details

Language :
English
ISSN :
2405805X
Volume :
8
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Synthetic and Systems Biotechnology
Publication Type :
Academic Journal
Accession number :
edsdoj.9b0299d06a9a44e998de76f413eafd35
Document Type :
article
Full Text :
https://doi.org/10.1016/j.synbio.2023.03.006