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Enhancement of the Chemiluminescence Response of Enzymatic Reactions by Plasmonic Surfaces for Biosensing Applications

Authors :
Biebele Abel
Babatunde Odukoya
Muzaffer Mohammed
Kadir Aslan
Source :
Nano Biomedicine and Engineering, Vol 7, Iss 3, Pp 92-101 (2015)
Publication Year :
2015
Publisher :
Tsinghua University Press, 2015.

Abstract

We report the enhancement of chemiluminescence response of horseradish peroxidase (HRP) in bioassays by plasmonic surfaces, which are comprised of (i) silver island films (SIFs) and (ii) metal thin films (silver, gold, copper, and nickel, 1 nm thick) deposited onto glass slides. A model bioassay, based on the interactions of avidin-modified HRP with a monolayer of biotinylated poly(ethylene-glycol)-amine, was employed to evaluate the ability of plasmonic surfaces to enhance chemiluminescence response of HRP. Chemiluminescence response of HRP in model bioassays were increased up to ~3.7-fold as compared to the control samples (i.e. glass slides without plasmonic nanoparticles), where the largest enhancement of the chemiluminescence response was observed on SIFs with high loading. These findings allowed us to demonstrate the use of SIFs (high loading) for the detection of a biologically relevant target protein (glial fibrillary acidic protein or GFAP), where the chemiluminescence response of the standard bioassay for GFAP was enhanced up to ~50% as compared to bioassay on glass slides.

Details

Language :
English
ISSN :
21505578
Volume :
7
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Nano Biomedicine and Engineering
Publication Type :
Academic Journal
Accession number :
edsdoj.9b8328d9c2bb4542a6f923514ea5053d
Document Type :
article
Full Text :
https://doi.org/10.5101/nbe.v7i3.p92-101