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Effects of ethephon on DNA methylation and gene expressions associated with shortened internodes in maize
- Source :
- Biotechnology & Biotechnological Equipment, Vol 32, Iss 1, Pp 30-40 (2018)
- Publication Year :
- 2018
- Publisher :
- Taylor & Francis Group, 2018.
-
Abstract
- In this study, the molecular mechanism underlying ethephon-induced shortened internodes in maize was investigated using Zhengdan958 maize variety. The ethephon treatment was performed by spraying 225 mL/ha Ethephon 40% SL onto the foliage at the eight-expanded-leaves stage. The differentially expressed genes in the young internodes under ethephon treatment were identified through methylation-sensitive amplified fragment length polymorphism (MSAP), start codon targeted polymorphism (SCoT) and cDNA-amplified fragment length polymorphism (AFLP) analyses. MSAP results showed the methylation sites were widely distributed in both the ethephon-treated (at 27.8%) and control plants (30.1%). This suggested that ethephon treatment modified the methylation patterns; with 3.0% of the sites being hyper-methylated and 7.3% demethylated compared with the control. Based on SCoT analysis, 148 transcript derived fragments (TDFs) were obtained in the ethephon-treated plants. Among them, 38 were up-regulated (25.7%) and 47 down-regulated (31.8%). cDNA-AFLP analysis using 70 primer pairs identified 1635 TDFs in the ethephon-treated and the control plants. Of these, 600 and 564 TDFs were up- and down-regulated by the ethephon treatment, respectively. BLASTX analysis on 50 (randomly selected) differentially expressed TDFs divided them into several categories based on their putative biological functions: signal transduction (6%), resistance-related (14%), energy and metabolism (22%), transcription (4%), cell apoptosis (2%), unknown functional proteins (42%) and unknown genes (10%). Our results revealed that ethephon treatment could induce DNA methylation variation principally by increasing the demethylation tendency. This is suggested to play roles in stress-defence genes expression regulation and the differentially expressed genes could be associated with shortened internodes in maize.
Details
- Language :
- English
- ISSN :
- 13102818 and 13143530
- Volume :
- 32
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Biotechnology & Biotechnological Equipment
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.9bc317c5686436eb7b36b834f38158d
- Document Type :
- article
- Full Text :
- https://doi.org/10.1080/13102818.2017.1386591