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Clinical Value of S-poly (T) plus Real-time PCR in Detection of Liver Cancer-related miR-199
- Source :
- Zhongliu Fangzhi Yanjiu, Vol 47, Iss 10, Pp 761-765 (2020)
- Publication Year :
- 2020
- Publisher :
- Magazine House of Cancer Research on Prevention and Treatment, 2020.
-
Abstract
- Objective To explore the advantages and disadvantages of S-poly(T) plus qRT-PCR and Poly (A) qRT-PCR methods in detecting peripheral blood serum miR-199. Methods S-poly(T) plus RT-PCR and Poly(A) qRT-PCR methods were used to detect the serum miR-199 in patients with liver cancer and healthy controls in three aspects of sensitivity, linearity and repeatability to evaluate their potential clinical value. Results The Ct values of S-poly(T) plus qRT-PCR method for miR-199 in healthy controls was significantly lower than that of Poly(A) qRT-PCR method (P < 0.0001); and the S-poly(T) plus qRT-PCR method had better linear correlation (P=0.0027) and repeatability (P=0.010). S-poly(T) plus qRT-PCR method had better sensitivity in detecting serum miR-199 in liver cancer patients comparing with Poly (A) qRT-PCR method. Conclusion In the detection of liver cancer serum miR-199, S-poly(T) plus qRT-PCR method is more sensitive, repeatable and stable than Poly(A) qRT-PCR.
Details
- Language :
- Chinese
- ISSN :
- 10008578
- Volume :
- 47
- Issue :
- 10
- Database :
- Directory of Open Access Journals
- Journal :
- Zhongliu Fangzhi Yanjiu
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.9c2ff433b83c4e8496ec31878896e6df
- Document Type :
- article
- Full Text :
- https://doi.org/10.3971/j.issn.1000-8578.2020.19.1618