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Laboratory diffracted x-ray blinking to monitor picometer motions of protein molecules and application to crystalline materials

Authors :
Tatsuya Arai
Rena Inamasu
Hiroki Yamaguchi
Daisuke Sasaki
Ayana Sato-Tomita
Hiroshi Sekiguchi
Kazuhiro Mio
Sakae Tsuda
Masahiro Kuramochi
Yuji C. Sasaki
Source :
Structural Dynamics, Vol 8, Iss 4, Pp 044302-044302-10 (2021)
Publication Year :
2021
Publisher :
AIP Publishing LLC and ACA, 2021.

Abstract

In recent years, real-time observations of molecules have been required to understand their behavior and function. To date, we have reported two different time-resolved observation methods: diffracted x-ray tracking and diffracted x-ray blinking (DXB). The former monitors the motion of diffracted spots derived from nanocrystals labeled onto target molecules, and the latter measures the fluctuation of the diffraction intensity that is highly correlated with the target molecular motion. However, these reports use a synchrotron x-ray source because of its high average flux, resulting in a high time resolution. Here, we used a laboratory x-ray source and DXB to measure the internal molecular dynamics of three different systems. The samples studied were bovine serum albumin (BSA) pinned onto a substrate, antifreeze protein (AFP) crystallized as a single crystal, and poly{2-(perfluorooctyl)ethyl acrylate} (PC8FA) polymer between polyimide sheets. It was found that not only BSA but also AFP and PC8FA molecules move in the systems. In addition, the molecular motion of AFP molecules was observed to increase with decreasing temperature. The rotational diffusion coefficients (DR) of BSA, AFP, and PC8FA were estimated to be 0.73 pm2/s, 0.65 pm2/s, and 3.29 pm2/s, respectively. Surprisingly, the DR of the PC8FA polymer was found to be the highest among the three samples. This is the first report that measures the molecular motion of a single protein crystal and polymer by using DXB with a laboratory x-ray source. This technique can be applied to any kind of crystal and crystalline polymer and provides atomic-order molecular information.

Subjects

Subjects :
Crystallography
QD901-999

Details

Language :
English
ISSN :
23297778
Volume :
8
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Structural Dynamics
Publication Type :
Academic Journal
Accession number :
edsdoj.9c3f53563d489c91231ad7292e49e4
Document Type :
article
Full Text :
https://doi.org/10.1063/4.0000112