Reconstructing a hydrogen-driven microbial metabolic network in Opalinus Clay rock

Hydrogen build-up in geological nuclear waste repositories poses risks, but it may be alleviated by H2 consumption by deep subsurface microbial communities. Here, the authors inject H2 in a borehole and use metagenomics and metaproteomics to identify a carbon cycle driven by autotrophic H2oxidizers.