Back to Search
Start Over
Improved 11α-hydroxycanrenone production by modification of cytochrome P450 monooxygenase gene in Aspergillus ochraceus
- Source :
- Acta Pharmaceutica, Vol 71, Iss 1, Pp 99-114 (2021)
- Publication Year :
- 2021
- Publisher :
- Sciendo, 2021.
-
Abstract
- Eplerenone is a drug that protects the cardiovascular system. 11α-Hydroxycanrenone is a key intermediate in eplerenone synthesis. We found that although the cytochrome P450 (CYP) enzyme system in Aspergillus ochraceus strain MF018 could catalyse the conversion of canrenone to 11α-hydroxycanrenone, its biocatalytic efficiency is low. To improve the efficiency of 11α-hydroxycanrenone production, the CYP monooxygenase-coding gene of MF018 was predicted and cloned based on whole-genome sequencing results. A recombinant A. ochraceus strain MF010 with the high expression of CYP monooxygenase was then obtained through homologous recombination. The biocatalytic rate of this recombinant strain reached 93 % at 60 h without the addition of organic solvents or surfactants and was 17–18 % higher than that of the MF018 strain. Moreover, the biocatalytic time of the MF010 strain was reduced by more than 30 h compared with that of the MF018 strain. These results show that the recombinant A. ochraceus strain MF010 can overcome the limitation of substrate biocatalytic efficiency and thus holds a high poten tial for application in the industrial production of eplerenone.
Details
- Language :
- English
- ISSN :
- 18469558
- Volume :
- 71
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Acta Pharmaceutica
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.9cd68267b658415192841c8e16a5f22d
- Document Type :
- article
- Full Text :
- https://doi.org/10.2478/acph-2021-0004