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Isolation, identification and Analysis of Drug Resistance of Salmonella Pul-lorum

Authors :
Liu Zhike
Tetiana Fotina
Roman Petrov
Zhanna Klishchova
Anatoliy Fotin
Source :
Ukrainian Journal of Veterinary and Agricultural Sciences, Vol 4, Iss 1, Pp 33-38 (2021)
Publication Year :
2021
Publisher :
Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, 2021.

Abstract

The article provides data on the isolation and identification of the pathogen S. Pullorum from pathological material of chickens. With further study of tinctorial, cultural-morphological and biological properties of the pathogen. The susceptibility of Salmonella pullorum to broad-spectrum antibacterial drugs such as cephalosporins and carbopenems was further studied to determine the drug of choice for improving treatment and prevention of avian bacterial diseases. In order to diagnose Salmonella pullorum (S. Pullorum) diarrhea accurately and analyze its drug resistance. In this study, the pathogen of a chicken suspected of S. Pullorum was isolation, PCR amplification and drug sensitivity analysis of the pathogen from in chicken farm in Xinxiang, north China. The results showed that the bacteria strain was diagnosed as S. Pullorum base on isolation and identification, Gram staining and biochemical identification of the bacteria. Antibacterial drugs sensitivity test confirmed that the bacteria was sensitive to ceftiofur, ceftriaxone, meropenem and kanamycin, and the effect of sensitive antibiotics was obvious in clinical treatment. Altogether, the present experiment revealed a detailed measure for S. Pullorum prevention and control and that achieved good clinical results, which laid a fundamental information for farmers and veterinary workers on eradication of S. Pullorum.

Details

Language :
English
ISSN :
26176149
Volume :
4
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Ukrainian Journal of Veterinary and Agricultural Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.9d5c6dcf1c2b4cabb856ac83f515c2d0
Document Type :
article
Full Text :
https://doi.org/10.32718/ujvas4-1.07