Prevalence and Clinical Relevance of Anti-FcϵRI Autoantibody in Crohn’s Disease

Yue Yin,1,&ast; Yusen Hu,2,&ast; Yanning Li,1 Xia Peng,1 Huanjin Liao,1 Wei Shen,3 Li Li1 1Department of Laboratory Medicine, Shanghai General Hospital, Shanghai, People’s Republic of China; 2Department of Gastroenterology, Shanghai General Hospital, Shanghai, People’s Republic of China; 3Department of Laboratory Medicine, Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, Shanghai, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Li Li, Department of Laboratory Medicine, Shanghai General Hospital, Shanghai, People’s Republic of China, Email annylish@126.com Wei Shen, Department of Laboratory Medicine, Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, Shanghai, People’s Republic of China, Email applessw@163.comBackground: Mast cells can be activated in various ways and were shown to be involved in the development of Crohn’s disease (CD). The diagnosis of CD is still challenging, and seeking novel biomarkers is a worthwhile endeavor.Methods: An indirect enzyme-linked immunosorbent assay (ELISA) was successfully established for semi-quantitative detection of IgG anti-Fc&epsiv;RI in serum using human Fc&epsiv;RIα coated microplates and an enzyme-labeled anti-human IgG as secondary antibodies. The optimal working conditions were explored, followed by conducting the method evaluation. The serum samples and clinical data of 117 CD patients and 75 healthy controls were collected. IgE was measured by the rate turbidity turbidimetry; IgG anti-IgE and IgG anti-Fc&epsiv;RI were detected by ELISA. IgG anti-pancreatic antibody (PAB) and anti-Saccharomyces cerevisiae antibody (ASCA) were determined by indirect immunofluorescence assay. Data were analyzed concerning the clinical characteristics.Results: IgG anti-Fc&epsiv;RI was an effective marker for CD (P < 0.001), but IgE and IgG anti-IgE (P = 0.089, 0.219, respectively) were not. There was a positive correlation between anti-IgE and anti-Fc&epsiv;RI (R = 0.380, P < 0.001). Anti-Fc&epsiv;RI positive patients behaved with higher disease activity [OR: 1.478 (1.200~1.821), P < 0.001], but were less likely to be located in L4 among Montreal classification [OR: 0.253 (0.077~0.837), P = 0.024]. Existing indicators, PAB and ASCA, behaved with high specificity (both > 95%) with low sensitivity (both < 30%). The combination of anti-Fc&epsiv;RI with existing markers significantly improved the diagnostic efficiency [AUC: 0.879 (0.831~0.928)].Conclusion: An ELISA for the detection of anti-Fc&epsiv;RI was established and validated, which may contribute to facilitating research on Crohn’s diseases. Anti-Fc&epsiv;RI positive CD patients were associated with higher disease activity indices, suggesting its potential value in the diagnosis and management of CD.Keywords: Crohn’s disease, anti-Fc&epsiv;RI, biomarkers, autoantibodies, mast cells