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Urotensin II promotes the proliferation and secretion of vascular endothelial growth factor in rat dermal papilla cells by activating the Wnt-β-catenin signaling pathway

Authors :
Congjuan Liao
Zhen Huang
Liuting Chen
Xiaorong Fan
Jun Peng
Xiaoqing Tan
Jianshe Yang
Xusheng Zhang
Source :
Italian Journal of Medicine, Vol 17, Iss 1 (2023)
Publication Year :
2023
Publisher :
PAGEPress Publications, 2023.

Abstract

Introduction. Urotensin II (U II) is a kind of active peptide with a variety of biological effects, such as promoting cell proliferation and endocrine effects. The aim of this study is to investigate the effect of urotensin II on the proliferation and secretion of vascular endothelial growth factor (VEGF) in cultured rat dermal papilla cells (DPCs), and to explore its molecular mechanism. Materials and Methods. We used the DPCs isolated from the thoracic aortas of Wistar-Kyoto rats to run the CCK8 and ELISA assay, RC-PCR and Western blotting techniques to identify the effect of Urotensin II on the proliferation and secretion of VEGF in DPCs, data were analyzed by one-way ANOVA or t-test. Results. U II can increase the mRNA expression of proliferation markers Ki67 and PCNA. In addition, the Wnt/β-catenin pathway was activated by U II, but Wnt inhibitor DKK1 reversed the effect of U II. Conclusions. U II promoted the proliferation and secretion of VEGF in rat DPCs through activation of the Wnt-β-catenin signaling pathway.

Details

Language :
English
ISSN :
18779344 and 18779352
Volume :
17
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Italian Journal of Medicine
Publication Type :
Academic Journal
Accession number :
edsdoj.b16bd35804784c1488bc3fa3756635df
Document Type :
article
Full Text :
https://doi.org/10.4081/itjm.2023.1607