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Acute Oral Toxicity and Genotoxicity Test and Evaluation of Cinnamomum camphora Seed Kernel Oil

Authors :
Pengbo Wang
Dongman Wan
Ting Peng
Yujing Yang
Xuefang Wen
Xianghui Yan
Jiaheng Xia
Qingwen Zhu
Ping Yu
Deming Gong
Zheling Zeng
Source :
Foods, Vol 12, Iss 2, p 293 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Cinnamomum camphora seed kernel oil (CCSKO) is one of the important natural medium chain triglycerides (MCT) resources, with more than 95.00% of medium chain fatty acids found in the world, and has various physiological effects. However, CCSKO has not been generally recognized as a safe oil or new food resource yet. The acute oral toxicity test and a standard battery of genotoxicity tests (mammalian erythrocyte micronucleus test, Ames test, and in vitro mammalian cell TK gene mutation test) of CCSKO as a new edible plant oil were used in the study. The results of the acute oral toxicity test showed that CCSKO was preliminary non-toxic, with an LD50 value higher than 21.5 g/kg body weight. In the mammalian erythrocyte micronucleus test, there was no concentration-response relationship between the dose of CCSKO and micronucleus value in polychromatic erythrocytes compared to the negative control group. No genotoxicity was observed in the Ames test in the presence or absence of S9 at 5000 μg/mL. In vitro mammalian cell TK gene mutation test showed that CCSKO did not induce in vitro mammalian cell TK gene mutation in the presence or absence of S9 at 5000 μg/mL. These results indicated that CCSKO is a non-toxic natural medium-chain oil.

Details

Language :
English
ISSN :
23048158
Volume :
12
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Foods
Publication Type :
Academic Journal
Accession number :
edsdoj.b1a2a44431ac460291b7d7ddd5945a2e
Document Type :
article
Full Text :
https://doi.org/10.3390/foods12020293