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Histomorphometric characteristics of immune cells in small intestine of pigs perorally immunized with vaccine candidate F18ac+ nonenterotoxigenic E. coli strain

Authors :
A. Kovsca Janjatovic
G. Lackovic
F. Bozic
D. Spoljaric
M. Popovic
H. Valpotic
N. Vijtiuk
Z. Pavicic
I. Valpotic
Source :
European Journal of Histochemistry, Vol 53, Iss 4, Pp e23-e23 (2009)
Publication Year :
2009
Publisher :
PAGEPress Publications, 2009.

Abstract

Colidiarrhea and colienterotoxemia caused by F4+ and/or F18+ enterotoxigenic E. coli (ETEC) strains are the most prevalent infections of suckling and weaned pigs. Here we tested the immunogenicity and protective effectiveness of attenuated F18ac+ non-ETEC vaccine candidate strain against challenge infection with F4ac+ ETEC strain by quantitative phenotypic analysis of small intestinal leukocyte subsets in weaned pigs. We also evaluated levamisole as an immune response modifier (IRM) and its adjuvanticity when given in the combination with the experimental vaccine. The pigs were parenterally immunized with either levamisole (at days -2, -1 and 0) or with levamisole and perorally given F18ac+ non-ETEC strain (at day 0), and challenged with F4ac+ ETEC strain 7 days later. At day 13 the pigs were euthanatized and sampled for immunohistological/histomorphometrical analyses. Lymphoid CD3+, CD45RA+, CD45RC+, CD21+, IgA+ and myeloid SWC3+ cell subsets were identified in jejunal and ileal epithelium, lamina propria and Peyer’s patches using the avidin-biotin complex method, and their numbers were determined by computer-assisted histomorphometry. Quantitative immunophenotypic analyses showed that levamisole treated pigs had highly increased numbers of jejunal CD3+, CD45RC+ and SWC3+ cells (p

Details

Language :
English
ISSN :
1121760X and 20388306
Volume :
53
Issue :
4
Database :
Directory of Open Access Journals
Journal :
European Journal of Histochemistry
Publication Type :
Academic Journal
Accession number :
edsdoj.b1b1bc370c6f42cba02f5db23c1e4405
Document Type :
article
Full Text :
https://doi.org/10.4081/ejh.2009.e23