Back to Search Start Over

Enzymatic response of ryegrass cellulose and hemicellulose valorization introduced by sequential alkaline extractions

Authors :
Shao-Fei Sun
Jing Yang
Da-Wei Wang
Hai-Yan Yang
Shao-Ni Sun
Zheng-Jun Shi
Source :
Biotechnology for Biofuels, Vol 14, Iss 1, Pp 1-12 (2021)
Publication Year :
2021
Publisher :
BMC, 2021.

Abstract

Abstract Background In view of the natural resistance of hemicelluloses in lignocellulosic biomass on bioconversion of cellulose into fermentable sugars, alkali extraction is considered as an effective method for gradually fractionating hemicelluloses and increasing the bioconversion efficiency of cellulose. In the present study, sequential alkaline extractions were performed on the delignified ryegrass material to achieve high bioconversion efficiency of cellulose and comprehensively investigated the structural features of hemicellulosic fractions for further applications. Results Sequential alkaline extractions removed hemicelluloses from cellulose-rich substrates and degraded part of amorphous cellulose, reducing yields of cellulose-rich substrates from 73.0 to 27.7% and increasing crystallinity indexes from 31.7 to 41.0%. Alkaline extraction enhanced bioconversion of cellulose by removal of hemicelluloses and swelling of cellulose, increasing of enzymatic hydrolysis from 72.3 to 95.3%. In addition, alkaline extraction gradually fractionated hemicelluloses into six fractions, containing arabinoxylans as the main polysaccharides and part of β-glucans. Simultaneously, increasing of alkaline concentration degraded hemicellulosic polysaccharides, which resulted in a decreasing their molecular weights from 67,510 to 50,720 g/mol. Conclusions The present study demonstrated that the sequential alkaline extraction conditions had significant effects on the enzymatic hydrolysis efficiency of cellulose and the investigation of the physicochemical properties of hemicellulose. Overall, the investigation the enzymatic hydrolysis efficiency of cellulose-rich substrates and the structural features of hemicelluloses from ryegrass will provide useful information for the efficient utilization of cellulose and hemicelluloses in biorefineries.

Details

Language :
English
ISSN :
17546834
Volume :
14
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Biotechnology for Biofuels
Publication Type :
Academic Journal
Accession number :
edsdoj.b29fb1e8736d44fc80be39632df8868e
Document Type :
article
Full Text :
https://doi.org/10.1186/s13068-021-01921-1