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LHH1, a novel antimicrobial peptide with anti-cancer cell activity identified from Lactobacillus casei HZ1

Authors :
Jun-Fang He
Du-Xin Jin
Xue-Gang Luo
Tong-Cun Zhang
Source :
AMB Express, Vol 10, Iss 1, Pp 1-15 (2020)
Publication Year :
2020
Publisher :
SpringerOpen, 2020.

Abstract

Abstract Antimicrobial peptides have been attracting increasing attention for their multiple beneficial effects. In present study, a novel AMP with a molecular weight of 1875.5 Da, was identified from the genome of Lactobacillus casei HZ1. The peptide, which was named as LHH1 was comprised of 16 amino acid residues, and its α-helix content was 95.34% when dissolved in 30 mM SDS. LHH1 exhibited a broad range of antimicrobial activities against Gram-positive bacteria and fungus. It could effectively inhibit Staphylococcus aureus with a minimum inhibitory concentration of 3.5 μM and showed a low hemolytic activity. The scanning electron microscope, confocal laser scanning microscope and flow cytometry results showed that LHH1 exerted its antibacterial activity by damaging the cell membrane of Staphylococcus aureus. Meanwhile, LHH1 also exhibited anti-cancer cell activities against several cancer cells via breaking the cell membrane of MGC803, HCT116 and C666-1 cancer cells.

Details

Language :
English
ISSN :
21910855
Volume :
10
Issue :
1
Database :
Directory of Open Access Journals
Journal :
AMB Express
Publication Type :
Academic Journal
Accession number :
edsdoj.b3691ba5fa4944dc801433448249ec33
Document Type :
article
Full Text :
https://doi.org/10.1186/s13568-020-01139-8