Effects of dietary supplementation of Lactobacillus plantarum and Bacillus subtilis on growth performance, survival, immune response, antioxidant capacity and digestive enzyme activity in olive flounder (Paralichthys olivaceus)

In present study, a 10-month feeding trial was carried out to investigate effects of dietary Lactobacillus plantarum and Bacillus subtilis supplementation singly on growth performance, innate immune response, antioxidant capacity and digestive enzyme activity of olive flounder under a commercial production environment. Two experimental groups fed basal diet with supplementation of L. plantarum (5.0 × 107 CFU/g diet) and B. subtilis (1.0 × 108 CFU/g diet) respectively, and control group fed with basal diet without probiotics supplement. At harvest, serum immune parameters, liver antioxidant indices and digestive enzyme activities in liver, stomach and intestines were measured. Fish fed with B. subtilis supplemented diet showed the highest growth rate which was significantly higher than L. plantarum supplemented group (P