Hygienic Practices and Level of Contamination in Toothbrushes at Mexican Homes in Guadalajara, Jalisco

In the present study, a Google Forms survey open questionnaire was applied to identify variables in hygiene habits that influence the contamination of toothbrushes in Mexican homes. A total of 275 questionnaires were validated, and 18 samples were analyzed to determine microbiology indicator groups (Mesophilic Aerobic Bacteria [MAB], Coliform Organisms [CO], and Yeast and Molds [Y/M]) with dilution and plate casting methods. Results were compared with data obtained from surveys, which were encoded to construct conglomerates. Interestingly, a higher microbial count was found in toothbrushes stored in cases (4.9 × 109 CFU/mL for MAB, 4.43 × 109 CFU/mL for CO, and 4.38 × 109 CFU/mL for Y/M), while the use and disposal of toothbrushes following recommendations of life expectancy limited bacterial growth (Klebsiella pneumoniae, Streptococcus pyogenes, and Pseudomonas, among others. Finally, most people do not have the habit of disinfecting their toothbrushes periodically. Further, the survey revealed that disinfection techniques applied by a limited group (14% of the respondents) are improvised and are not standardized with the objective of disinfection.