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Recombinase Polymerase Amplification assay for detection of the British root-knot nematode, Meloidogyne artiellia
- Source :
- Journal of Nematology, Vol 56, Iss 1, Pp 11788-1523 (2024)
- Publication Year :
- 2024
- Publisher :
- Sciendo, 2024.
-
Abstract
- Recombinase polymerase amplification (RPA) is an isothermal in vitro nucleic acid amplification technique that has been adopted for simple, robust, rapid, reliable diagnostics of nematodes. In this study, the real-time RPA assay and RPA assay combined with lateral flow dipsticks (LF-RPA) have been developed targeting the ITS rRNA gene of the British root-knot nematode, Meloidogyne artiellia. The assay provided specific and rapid detection of this root-knot nematode species from crude nematode extracts without a DNA extraction step with a sensitivity of 0.125 second-stage juvenile (J2) specimen per a reaction tube for real-time RPA during 11 min and a sensitivity of 0.5 J2 specimens per a reaction tube for LF-RPA during 25 min. The RPA assays were validated with a wide range of non-target root-knot nematodes. The LF-RPA assay has great potential for nematode diagnostics in the laboratory having minimal available equipment.
Details
- Language :
- English
- ISSN :
- 2640396X and 20240023
- Volume :
- 56
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of Nematology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.b93a5e4c2df4ccaa721ec7fc8543413
- Document Type :
- article
- Full Text :
- https://doi.org/10.2478/jofnem-2024-0023