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A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics

Authors :
Pan Fang
Yanlong Ji
Ivan Silbern
Carmen Doebele
Momchil Ninov
Christof Lenz
Thomas Oellerich
Kuan-Ting Pan
Henning Urlaub
Source :
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Publication Year :
2020
Publisher :
Nature Portfolio, 2020.

Abstract

Comprehensive quantitative profiling of intact glycopeptides remains technically challenging. To address this, the authors here develop an integrated quantitative glycoproteomic workflow, including optimized sample preparation, multiplexed quantification and a dedicated data processing tool.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
11
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.b98847062cd64d348c1ad9038cab217f
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-020-19052-w