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Whole Genome Sequence of the Heterozygous Clinical Isolate Candida krusei 81-B-5

Authors :
Christina A. Cuomo
Terrance Shea
Bo Yang
Reeta Rao
Anja Forche
Source :
G3: Genes, Genomes, Genetics, Vol 7, Iss 9, Pp 2883-2889 (2017)
Publication Year :
2017
Publisher :
Oxford University Press, 2017.

Abstract

Candida krusei is a diploid, heterozygous yeast that is an opportunistic fungal pathogen in immunocompromised patients. This species also is utilized for fermenting cocoa beans during chocolate production. One major concern in the clinical setting is the innate resistance of this species to the most commonly used antifungal drug fluconazole. Here, we report a high-quality genome sequence and assembly for the first clinical isolate of C. krusei, strain 81-B-5, into 11 scaffolds generated with PacBio sequencing technology. Gene annotation and comparative analysis revealed a unique profile of transporters that could play a role in drug resistance or adaptation to different environments. In addition, we show that, while 82% of the genome is highly heterozygous, a 2.0 Mb region of the largest scaffold has undergone loss of heterozygosity. This genome will serve as a reference for further genetic studies of this pathogen.

Details

Language :
English
ISSN :
21601836
Volume :
7
Issue :
9
Database :
Directory of Open Access Journals
Journal :
G3: Genes, Genomes, Genetics
Publication Type :
Academic Journal
Accession number :
edsdoj.b9f8280ffa2c4aabbe2af9335d876f56
Document Type :
article
Full Text :
https://doi.org/10.1534/g3.117.043547