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High-depth spatial transcriptome analysis by photo-isolation chemistry

Authors :
Mizuki Honda
Shinya Oki
Ryuichi Kimura
Akihito Harada
Kazumitsu Maehara
Kaori Tanaka
Chikara Meno
Yasuyuki Ohkawa
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

Spatial analysis of RNAseq data is important. Here the authors report a method for transcriptome profiling combined with photo-isolation chemistry to allow determination of expression profiles specifically from photo-irradiated regions of interest which they use in mouse brains and embryonic tissues.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.bb1fc3bbebd6485c9ec92dde46e117b8
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-021-24691-8