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The RFamide receptor DMSR-1 regulates stress-induced sleep in C. elegans

Authors :
Michael J Iannacone
Isabel Beets
Lindsey E Lopes
Matthew A Churgin
Christopher Fang-Yen
Matthew D Nelson
Liliane Schoofs
David M Raizen
Source :
eLife, Vol 6 (2017)
Publication Year :
2017
Publisher :
eLife Sciences Publications Ltd, 2017.

Abstract

In response to environments that cause cellular stress, animals engage in sleep behavior that facilitates recovery from the stress. In Caenorhabditis elegans, stress-induced sleep(SIS) is regulated by cytokine activation of the ALA neuron, which releases FLP-13 neuropeptides characterized by an amidated arginine-phenylalanine (RFamide) C-terminus motif. By performing an unbiased genetic screen for mutants that impair the somnogenic effects of FLP-13 neuropeptides, we identified the gene dmsr-1, which encodes a G-protein coupled receptor similar to an insect RFamide receptor. DMSR-1 is activated by FLP-13 peptides in cell culture, is required for SIS in vivo, is expressed non-synaptically in several wake-promoting neurons, and likely couples to a Gi/o heterotrimeric G-protein. Our data expand our understanding of how a single neuroendocrine cell coordinates an organism-wide behavioral response, and suggest that similar signaling principles may function in other organisms to regulate sleep during sickness.

Details

Language :
English
ISSN :
2050084X
Volume :
6
Database :
Directory of Open Access Journals
Journal :
eLife
Publication Type :
Academic Journal
Accession number :
edsdoj.bb8618cb55f419390d4dc38606a1b29
Document Type :
article
Full Text :
https://doi.org/10.7554/eLife.19837