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WRKY33 negatively regulates anthocyanin biosynthesis and cooperates with PHR1 to mediate acclimation to phosphate starvation

Authors :
Han Tao
Fei Gao
Linying Li
Yuqing He
Xueying Zhang
Mengyu Wang
Jia Wei
Yao Zhao
Chi Zhang
Qiaomei Wang
Gaojie Hong
Source :
Plant Communications, Vol 5, Iss 5, Pp 100821- (2024)
Publication Year :
2024
Publisher :
Elsevier, 2024.

Abstract

Anthocyanin accumulation is acknowledged as a phenotypic indicator of phosphate (Pi) starvation. However, negative regulators of this process and their molecular mechanisms remain largely unexplored. In this study, we demonstrate that WRKY33 acts as a negative regulator of phosphorus-status-dependent anthocyanin biosynthesis. WRKY33 regulates the expression of the gene encoding dihydroflavonol 4-reductase (DFR), a rate-limiting enzyme in anthocyanin production, both directly and indirectly. WRKY33 binds directly to the DFR promoter to repress its expression and also interferes with the MBW complex through interacting with PAP1 to indirectly influence DFR transcriptional activation. Under −Pi conditions, PHR1 interacts with WRKY33, and the protein level of WRKY33 decreases; the repression of DFR expression by WRKY33 is thus attenuated, leading to anthocyanin accumulation in Arabidopsis. Further genetic and biochemical assays suggest that PHR1 is also involved in regulating factors that affect WRKY33 protein turnover. Taken together, our findings reveal that Pi starvation represses WRKY33, a repressor of anthocyanin biosynthesis, to finely tune anthocyanin biosynthesis. This “double-negative logic” regulation of phosphorus-status-dependent anthocyanin biosynthesis is required for the maintenance of plant metabolic homeostasis during acclimation to Pi starvation.

Details

Language :
English
ISSN :
25903462 and 75646439
Volume :
5
Issue :
5
Database :
Directory of Open Access Journals
Journal :
Plant Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.bc3c509db756464398f8c34a019c218b
Document Type :
article
Full Text :
https://doi.org/10.1016/j.xplc.2024.100821