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A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

Authors :
Mohsen Mohammadniaei
Ming Zhang
Jon Ashley
Ulf Bech Christensen
Lennart Jan Friis-Hansen
Rasmus Gregersen
Jan Gorm Lisby
Thomas Lars Benfield
Finn Erland Nielsen
Jens Henning Rasmussen
Ellen Bøtker Pedersen
Anne Christine Rye Olinger
Lærke Tørring Kolding
Maryam Naseri
Tao Zheng
Wentao Wang
Jan Gorodkin
Yi Sun
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

The reliance on enzymes in SARS-CoV-2 RNA detection imposes limits on transport and storage conditions. Here the authors use non-enzymatic isothermal amplification to detect RNA with no need for reverse transcription.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.bd06ee023a57436bb5b1ecd0d8bd16db
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-021-25387-9