Back to Search Start Over

RNF20 Regulates Oocyte Meiotic Spindle Assembly by Recruiting TPM3 to Centromeres and Spindle Poles

Authors :
Liying Wang
Chao Liu
Li Li
Huafang Wei
Wei Wei
Qiuxing Zhou
Yinghong Chen
Tie‐Gang Meng
Renjie Jiao
Zhen‐Bo Wang
Qing‐Yuan Sun
Wei Li
Source :
Advanced Science, Vol 11, Iss 13, Pp n/a-n/a (2024)
Publication Year :
2024
Publisher :
Wiley, 2024.

Abstract

Abstract Previously a ring finger protein 20 (RNF20) is found to be essential for meiotic recombination and mediates H2B ubiquitination during spermatogenesis. However, its role in meiotic division is still unknown. Here, it is shown that RNF20 is localized at both centromeres and spindle poles, and it is required for oocyte acentrosomal spindle organization and female fertility. RNF20‐depleted oocytes exhibit severely abnormal spindle and chromosome misalignment caused by defective bipolar organization. Notably, it is found that the function of RNF20 in spindle assembly is not dependent on its E3 ligase activity. Instead, RNF20 regulates spindle assembly by recruiting tropomyosin3 (TPM3) to both centromeres and spindle poles with its coiled‐coil motif. The RNF20‐TPM3 interaction is essential for acentrosomal meiotic spindle assembly. Together, the studies uncover a novel function for RNF20 in mediating TPM3 recruitment to both centromeres and spindle poles during oocyte spindle assembly.

Details

Language :
English
ISSN :
21983844
Volume :
11
Issue :
13
Database :
Directory of Open Access Journals
Journal :
Advanced Science
Publication Type :
Academic Journal
Accession number :
edsdoj.bd0eef6c4c6d4ade8509c2bbda8550d1
Document Type :
article
Full Text :
https://doi.org/10.1002/advs.202306986