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Phenolic compounds and antifungal activity of ethyl acetate extract and methanolic extract from Capsicum chinense Jacq. ripe fruit

Authors :
L. S. Santos
C. C. Fernandes
A. L. B. Dias
E. L. Souchie
M. L. D. Miranda
Source :
Brazilian Journal of Biology, Vol 84 (2022)
Publication Year :
2022
Publisher :
Instituto Internacional de Ecologia, 2022.

Abstract

Abstract Food loss due to contamination caused by fungi has much impact on agriculture and leads to significant economic losses. Synthetic and natural fungicides have been used for avoiding losses of several food products due to fungal contamination. As a result, species of the genus Capsicum have been used for preserving food because of their chemical compounds with antifungal activity. Therefore, this study aimed at identifying some phenolic compounds found in both ethyl acetate extract (EAE) and methanolic extract (ME) from habanero pepper (C. chinense) ripe fruit by liquid chromatography tandem mass spectrometry with electrospray ionization (LC-ESI-MS/MS) and at evaluating their antifungal activities against fungi Sclerotinia sclerotiorum, Rhizopus stolonifer and Colletotrichum gloeosporioides. Extracts resulted from a sequential process of maceration. Antifungal activity was evaluated by the disk diffusion method (DDM) at the following doses of both diluted extracts: 25 µL, 50 µL, 100 µL and 200 µL. The chemical analysis showed that there were protocatechuic acid, gentisic acid, vanillic acid, kaempferol-3-O-robinobiosideo and naringenin in both extracts. EAE showed high inhibition of mycelial growth at both doses 100µL and 200µL against the three fungi while methanolic exhibited weak activity even at the highest dose under investigation. However, further in-depth studies are needed to reinforce their uses and practical applications to the agricultural field.

Details

Language :
English
ISSN :
16784375 and 15196984
Volume :
84
Database :
Directory of Open Access Journals
Journal :
Brazilian Journal of Biology
Publication Type :
Academic Journal
Accession number :
edsdoj.bdc8160aa8034362b0f0d9fe82814dad
Document Type :
article
Full Text :
https://doi.org/10.1590/1519-6984.258084