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Copy Number Variation of the PIGY Gene in Sheep and Its Association Analysis with Growth Traits

Authors :
Ziting Feng
Xinyu Li
Jie Cheng
Rui Jiang
Ruolan Huang
Dingchuan Wang
Yongzhen Huang
Li Pi
Linyong Hu
Hong Chen
Source :
Animals, Vol 10, Iss 4, p 688 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Copy number variation (CNV) is a type of genomic variation with an important effect on animal phenotype. We found that the PIGY gene contains a 3600 bp copy number variation (CNV) region located in chromosome 6 of sheep (Oar_v4.0 36,121,601–36,125,200 bp). This region overlaps with multiple quantitative trait loci related to phenotypes like muscle density and carcass weight. Therefore, in this study, the copy number variation of the PIGY gene was screened in three Chinese sheep breeds, namely, Chaka sheep (CKS, May of 2018, Wulan County, Qinghai Province, China), Hu sheep (HS, May of 2015, Mengjin County, Henan Province, China), and small-tailed Han sheep (STHS, May of 2016, Yongjing, Gansu Province, China). Association analyses were performed on the presence of CNV and sheep body size traits. We used real-time quantitative PCR (qPCR) to detect the CNV for association analysis. According to the results, the loss-type CNV was more common than other types in the three breeds (global average: loss = 61.5%, normal = 17.5%, and gain = 21.0%). The association analysis also showed significant effects of the PIGY gene CNV on body weight, chest circumference, and circumference of the cannon bone of sheep. Sheep with gain-type CNV had better growth traits than those with other types. The results indicate a clear relationship between the PIGY gene CNV and growth traits of sheep, suggesting the use of CNV as a new molecular breeding marker.

Details

Language :
English
ISSN :
20762615 and 83781250
Volume :
10
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Animals
Publication Type :
Academic Journal
Accession number :
edsdoj.f0836eaa00df4aac83781250849db0b9
Document Type :
article
Full Text :
https://doi.org/10.3390/ani10040688