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Diet-Induced Obesity and Ghrelin Effects on Pituitary Gonadotrophs: Immunohistomorphometric Study in Male Rats

Diet-Induced Obesity and Ghrelin Effects on Pituitary Gonadotrophs: Immunohistomorphometric Study in Male Rats

Authors :
Ristic Natasa
Stevanovic Darko
Nesic Dejan
Ajdzanovic Vladimir
Rakocevic Rastko
Jaric Ivana
Milosevic Verica
Source :
Cell Journal, Vol 17, Iss 4, Pp 711-719 (2016)
Publication Year :
2016
Publisher :
Royan Institute (ACECR), Tehran, 2016.

Abstract

Objective: The close relationship between energy metabolism, nutritional state, and reproductive physiology suggests that nutritional and metabolic disorders can disrupt normal reproductive function and fertility. Considering the importance of leptin and ghrelin effects in regulation of the hypothalamic-pituitary-gonadal axis, the objective of this study was to investigate the influence of obesity and centrally applied ghrelin on immunohistochemical appearance and quantitative morphology of the pituitary follicle-stimulating hormone (FSH) and luteinizing hormone (LH) producing cells in adult male rats. Materials and Methods: In this experimental study, animals were given two different diets: normal-fat (NF) and high-fat (HF), for 4 weeks, corresponding to normal and positive energy balance (n=2×14), respectively. Each group was subsequently divided into two subgroups (n=7) receiving intracerebroventricular (ICV) injections of either ghrelin [G, 1 μg/5 μL phosphate buffered saline (PBS)] or vehicle (5 μL PBS, control group) every 24 hours for five consecutive days. Results: Morphometric analyses showed that in HF control group, the percentage of FSH cells per unit volume of total pituitary gland tissue (in μm3), i.e. volume density (Vvc), was increased (P

Details

Language :
English
ISSN :
22285806 and 22285814
Volume :
17
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Cell Journal
Publication Type :
Academic Journal
Accession number :
edsdoj.f0d72a514f5442c6b7caf88f3b33a968
Document Type :
article