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Evaluation of the Therapeutic Potential of Traditionally-Used Natural Plant Extracts to Inhibit Proliferation of a HeLa Cell Cancer Line and Replication of Human Respiratory Syncytial Virus (hRSV)

Authors :
Ellie N. Brill
Natalie G. Link
Morgan R. Jackson
Alea F. Alvi
Jacob N. Moehlenkamp
Morgan B. Beard
Adam R. Simons
Linden C. Carson
Ray Li
Breckin T. Judd
Max N. Brasseale
Emily P. Berkman
Riley K. Park
Sedna Cordova-Hernandez
Rebecca Y. Hoff
Caroline E. Yager
Meredith C. Modelski
Milica Nenadovich
Dhruvi Sisodia
Clayton J. Reames
Andreas G. Geranios
Sean T. Berthrong
Anne M. Wilson
Ashlee H. Tietje
Christopher C. Stobart
Source :
Biology, Vol 13, Iss 9, p 696 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Traditional approaches employing natural plant products to treat a wide array of ailments have been documented and described for thousands of years. However, there remains limited scientific study of the therapeutic potential or effectiveness of ethnobotanical applications. Increases in the incidence of cancer and emerging infectious diseases demonstrate a growing need for advances in the development of therapeutic options. In this study, we evaluate the therapeutic potential of aqueous extracts prepared from four plants, purple aster (Symphyotrichum novae-angliae (L.) Nemsom), common sage (Salvia lyrata (L.)), northern spicebush (Lindera benzoin (L.) Blume), and lamb’s ear (Stachys byzantina (K.) Koch)) traditionally used in Native American medicine in Indiana, USA. Using a combination of cytotoxicity assays, immunofluorescence microscopy, and antiviral assays, we found that sage and spicebush extracts exhibit cytotoxic and antiproliferative effects on HeLa cell proliferation and that sage, spicebush, and aster extracts were capable of significantly inhibiting human respiratory syncytial virus (hRSV), a major respiratory pathogen of infants and the elderly. Chemical analysis of the four extracts identified four major compounds which were subsequently evaluated to identify the responsible constituents in the extracts. While none of the identified compounds were shown to induce significant impacts on HeLa cell proliferation, two of the compounds, (1S)-(-)-Borneol and 5-(hydroxymethyl)-furfural, identified in sage and spicebush, respectively, were shown to have antiviral activities. Our data suggest that several of the extracts tested exhibited either anti-proliferative or antiviral activity supporting future further analysis.

Details

Language :
English
ISSN :
20797737
Volume :
13
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Biology
Publication Type :
Academic Journal
Accession number :
edsdoj.f19f7255ae004bfaabf7d12912fe25dc
Document Type :
article
Full Text :
https://doi.org/10.3390/biology13090696