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MiR-1278 targets CALD1 and suppresses the progression of gastric cancer via the MAPK pathway
- Source :
- Open Medicine, Vol 18, Iss 1, Pp 52790-60 (2023)
- Publication Year :
- 2023
- Publisher :
- De Gruyter, 2023.
-
Abstract
- This study aimed to investigate the interaction between miR-1278 and Caldesmon (CALD1) in gastric cancer (GC) and the regulatory mechanism. In both GC cells and tissues, the levels of CALD1, miR-1278, migration-related markers (E-cadherin, N-cadherin, and Snail), and MAPK signaling pathway-related proteins were clarified using quantitative real-time PCR and western blotting analyses. The effects of miR-1278 and CALD1 on GC cell viability and migration were analyzed using CCK-8 and Transwell assays, respectively. The targeting effect of miR-1278 on CALD1 was investigated using bioinformatics prediction and a dual luciferase reporter assay. The effect of miR-1278 on tumor growth was estimated in vivo using a tumor xenograft assay. In GC, miR-1278 expression decreased, whereas CALD1 was highly expressed. Transfecting an miR-1278 mimic into cells inhibited the viability as well as migration of GC cells, and suppressed Ras, phosphorylated (p)-P38, and p-ERK1/2 protein levels. Moreover, miR-1278 targeted and negatively regulated CALD1 expression. CALD1 overexpression promoted GC cell survival and migration and activated the MAPK pathway. Treatment with an miR-1278 mimic partially rescued the changes caused by CALD1 overexpression. Overall, our study revealed that miR-1278 suppresses the malignant behavior of GC cells by targeting CALD1 and regulating the MAPK pathway.
- Subjects :
- mir-1278
cald1
gastric cancer
migration
Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 23915463
- Volume :
- 18
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Open Medicine
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.f20e9c84f64d466fad9766b77ece04d4
- Document Type :
- article
- Full Text :
- https://doi.org/10.1515/med-2023-0776