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MiRNA Profiling in Plasma Neural-Derived Small Extracellular Vesicles from Patients with Alzheimer’s Disease

Authors :
Maria Serpente
Chiara Fenoglio
Marianna D’Anca
Marina Arcaro
Federica Sorrentino
Caterina Visconte
Andrea Arighi
Giorgio G. Fumagalli
Laura Porretti
Alessandra Cattaneo
Miriam Ciani
Roberta Zanardini
Luisa Benussi
Roberta Ghidoni
Elio Scarpini
Daniela Galimberti
Source :
Cells, Vol 9, Iss 6, p 1443 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Small extracellular vesicles (EVs) are able to pass from the central nervous system (CNS) into peripheral blood and contain molecule markers of their parental origin. The aim of our study was to isolate and characterize total and neural-derived small EVs (NDEVs) and their micro RNA (miRNA) cargo in Alzheimer’s disease (AD) patients. Small NDEVs were isolated from plasma in a population consisting of 40 AD patients and 40 healthy subjects (CTRLs) using high throughput Advanced TaqMan miRNA OpenArrays®, which enables the simultaneous determination of 754 miRNAs. MiR-23a-3p, miR-223-3p, miR-100-3p and miR-190-5p showed a significant dysregulation in small NDEVs from AD patients as compared with controls (1.16 ± 0.49 versus 7.54 ± 2.5, p = 0.026; 9.32 ± 2.27 versus 0.66 ± 0.18, p p < 0.0001 and 2.9 ± 1.2 versus 1.93 ± 0.9, p < 0.05, respectively). A further validation analysis confirmed that miR-23a-3p, miR-223-3p and miR-190a-5p levels in small NDEVs from AD patients were significantly upregulated as compared with controls (p = 0.008; p = 0.016; p = 0.003, respectively) whereas miR-100-3p levels were significantly downregulated (p = 0.008). This is the first study that carries out the comparison between total plasma small EV population and NDEVs, demonstrating the presence of a specific AD NDEV miRNA signature.

Details

Language :
English
ISSN :
20734409
Volume :
9
Issue :
6
Database :
Directory of Open Access Journals
Journal :
Cells
Publication Type :
Academic Journal
Accession number :
edsdoj.f2e20384517e4d0896de94de00a8e6eb
Document Type :
article
Full Text :
https://doi.org/10.3390/cells9061443