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Comparative Antioxidant Activity and Untargeted Metabolomic Analyses of Sour Cherry Cultivars Based on Ultra-Performance–Time of Flight–Mass Spectrometry

Authors :
Prabhjot Kaur
Ahmed G. Darwish
Islam El-Sharkawy
Ashutosh Singh
Jayasankar Subramanian
Source :
Plants, Vol 13, Iss 11, p 1511 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

This study was conducted for the comparative analysis of antioxidant activity and untargeted metabolomics of dark- and light-colored sour cherry cultivars grown in Canada. Based on our previous study, we selected four cultivars—‘Heimann R’, ‘Gorsemska’, V70142, and ‘Montmorency’—to determine the untargeted metabolites and their role in antioxidant activities. A total of 473 metabolites were identified from four sour cherry genotypes using UPLC–ToF–MS. Untargeted metabolomics revealed the dominant chemical groups present in sour cherries. PCA showed that the diversity in sour cherry metabolites was due to the genotype differences indicating iditol, malic acid, chlorobenzene, 2-mercaptobenzothiazole, and pyroglutamic acid as the predominant contributors. The variable importance in the projection (VIP > 1.0) in partial least-squares–discriminant analysis described 20 biomarker metabolites representing the cherry metabolome profiles. A heatmap of Pearson’s correlation analysis between the 20 biomarker metabolites and antioxidant activities identified seven antioxidant determinants that displayed the highest correlations with different types of antioxidant activities. TPC and TAC were evaluated using the Folin–Ciocalteu method. The total antioxidant activity was performed using three different assays (ABTS, FRAP, and DPPH). This study of correlating metabolomics and antioxidant activities elucidated that the higher nutritional value and biological functions of sour cherry genotypes can be useful for the development of nutraceutical and functional foods.

Details

Language :
English
ISSN :
22237747
Volume :
13
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Plants
Publication Type :
Academic Journal
Accession number :
edsdoj.f3df786968b443a8a61903763bd18b6
Document Type :
article
Full Text :
https://doi.org/10.3390/plants13111511